![]() ![]() For example, the antigen-binding efficiencies of an anti-C-reactive protein antibody-immobilized gold sensor surface 10 and anti-ferritin antibody-immobilized latex particles 11 were improved by the co-immobilization of densely packed PEG layers, consisting of sulfhydryl-terminated PEGs and oligoamine-terminated PEGs. Furthermore, in our recent studies, we discovered that PEGylation improves the functioning of immobilized proteins on solid surfaces and particles. Because of the nonionic properties, hydrophilicity and large steric-exclusion effect of PEG, 5 PEGylated surfaces and nano- and microscale particles show excellent non-fouling properties with various molecules 6 and high-dispersion stabilities, 7, 8, 9 respectively. Poly(ethylene glycol) (PEG) is known as an excellent blocking agent. In the development of high-performance materials for biosensing and bioseparation, an effective blocking treatment is important to decrease nonspecific adsorption onto the surfaces of protein-immobilized substrates and to increase the dispersion stability of substrate particles. 1, 2, 3, 4 Protein-immobilized substrates, such as column packings and magnetic beads, have also been used as selective materials that can rapidly and simply separate target biomolecules in solution. Many kinds of sensors have been based on changes in colorimetric, fluorometric or luminometric signals derived from the immunoreactions of protein-immobilized sensor chips and particles. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility, making them an ideal choice for commercial supply.Protein-immobilized substrates have been widely used in several applications for biosensing and bioseparation. Our manufacturing sites are ISO 13485–certified. This process can be automated using any of our KingFisher sample purification systems The 2.8-μm magnetic beads are ideal for high-throughput enrichment. The kit is designed for and tested with cultured yeast and mammalian cells and is compatible with tissue, insect, bacterial, and other lysates.Īutomation-ready for use with KingFisher purification systems Magnetic bead–based separation offers easy handlingĬaptured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag magnet due to the magnetic properties of the Dynabeads M-270 Epoxy beads. The protocol includes coupling, sample preparation (detergent and cryolysis methods), co-IP, and elution and is compatible with western blot/silver stain, Coomassie stain, mass spectrometry, and extraction of nucleic acids from isolated DNA/RNA binding protein complexes. Target specific antibody is required for coupling, but not included. All kit components are analytical grade and compatible with protease and phosphatase inhibitors. The kit includes eight different binding, washing, and elution buffers, in addition to Dynabeads M-270 Epoxy beads, to enable purification of complex protein structures of any size. The supplied buffers are optimized to give consistent and reliable results. Dynabeads M-270 Epoxy beads exhibit ultra-low background binding and do not require blocking before use. With this kit, antibodies are covalently coupled to Dynabeads M-270 Epoxy beads, minimizing the risk of bound antibodies contaminating the final eluate. Robustness-covalent antibody coupling to the Dynabeads magnetic beads avoids co-elution of the antibody with the target proteins.Flexibility-allows coupling of any antibody of your choice.Stabile-gentle separation technology preserves native protein conformations and large protein complexes.Convenience-contains optimized buffers and epoxy-activated Dynabeads magnetic beads for efficient co-IP.In addition to epoxy-activated Dynabeads magnetic beads, the kit includes the buffers needed for efficient antibody coupling, gentle washing, and elution of the co-isolated protein complexes.īenefits of Dynabeads Co-Immunoprecipitation Kit include: The coupled beads and supplied buffers can then be used for co-immunoprecipitation (co-IP) of proteins, intact protein complexes, or intact protein-nucleic acid complexes. The Dynabeads™ Co-Immunoprecipitation Kit is intended for the coupling of an antibody to the surface of Dynabeads™ M-270 Epoxy beads. ![]()
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